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Numéro d'article:
21933063
Nanopore Sequencing: An Introduction
Numéro d'article:
21933063
CHF 120
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An introductory text and lab manual on nanopore sequencing for undergraduate students, graduate students and research scientists.
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Ce qui se démarque
Real-Time Analysis
Nanopore sequencing enables immediate data acquisition, allowing for rapid analysis and faster decision-making in research and clinical applications.
Long Reads
It offers the capability to generate ultra-long reads, improving genome assembly and providing greater insights into complex genomic regions.
Portability
The compact design of nanopore sequencers allows for field-based sequencing, supporting on-site investigations and making genomic research more accessible.
Détails du produit
- Introductory text and laboratory manual for undergraduate courses
- Useful for graduate students and research scientists for nanopore sequencing
- Clear explanations of principles and instructional material written by experts
- Serves as a textbook and lab manual at Harvard University for biology courses
- Intensifies intellectual experience and explores biology as a field of concentration
- Pre-empts complaint about taking physics or math while majoring in biology by demonstrating the range of topics and skills needed in subsequent courses
| Publisher | World Scientific Publishing Company |
| Publication date | April 19, 2019 |
| Language | English |
| Print length | 216 pages |
| ISBN-10 | 9813270608 |
| ISBN-13 | 978-9813270602 |
| Item Weight | 15.7 ounces (445.1 grams) |
| Dimensions | 6 x 0.56 x 9 inches (15.2 x 1.4 x 22.9 cm) |
À qui est-ce destiné ?
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Researchers
Ideal for researchers seeking a comprehensive understanding of nanopore sequencing technology and its applications in genomics.
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Students
Perfect for students in molecular biology or genomics courses who need an accessible introduction to sequencing technologies.
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Bioinformatics Professionals
Beneficial for bioinformatics experts looking to expand their knowledge on nanopore data analysis and interpretation techniques.
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Beginners in Biology
Not ideal for complete beginners without background knowledge in genetics or molecular biology concepts.
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Advanced Users
May not satisfy experienced professionals seeking advanced discussions or intricate technical details beyond introductory content.
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Budget-Conscious Learners
Could be unsuitable for those on a tight budget, as it may involve costs related to additional materials or resources.
DESCRIPTION DU PRODUIT
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Questions et réponses des clients
-
question:
What is nanopore sequencing?
répondre: Nanopore sequencing is a cutting-edge DNA and RNA sequencing technology that enables real-time analysis of nucleic acids. It involves passing DNA strands through a nanopore, where changes in ionic current signal the sequence of bases. This method allows for long-read sequencing, making it ideal for studying complex genomes, detecting structural variants, and analyzing RNA transcripts. Researchers and clinicians utilize nanopore sequencing for applications like personalized medicine, infectious disease identification, and genomic research, benefiting from its rapid results and portability. -
question:
How does nanopore sequencing compare to traditional sequencing methods?
répondre: Nanopore sequencing differs from traditional methods, such as Sanger or Illumina sequencing, primarily in its ability to produce longer reads and perform real-time analysis. While traditional methods often require extensive laboratory setups and analysis times, nanopore sequencing can be conducted on portable devices, allowing for field applications. This makes it particularly useful in remote settings or during outbreaks, where timely genomic information is critical for public health responses. -
question:
What are the advantages of using nanopore sequencing?
répondre: The key advantages of nanopore sequencing include its ability to generate long reads, enabling comprehensive genomic analysis, and real-time data output, facilitating immediate insights. Additionally, its portability allows use in diverse environments, from research labs to field diagnostics. This versatility supports applications ranging from ecological studies to rapid pathogen detection during health crises, offering a significant edge over other sequencing technologies. -
question:
What types of samples can be used with nanopore sequencing?
répondre: Nanopore sequencing is compatible with a wide variety of biological samples, including whole blood, tissue biopsies, and microbial cultures. Its flexibility enables researchers to sequence genomic DNA, cDNA from RNA transcripts, and even complex metagenomic samples. This adaptability makes it an excellent choice for diverse applications in genomics, transcriptomics, and microbiomics, allowing scientists to explore intricate biological questions across different organisms. -
question:
Is the nanopore sequencing process complex?
répondre: The nanopore sequencing process is relatively straightforward, typically involving sample preparation, loading the DNA onto a flow cell, and then allowing the sequencing device to read the genetic material in real-time. Minimal hands-on time is required, making it accessible even for labs without extensive sequencing experience. It’s particularly advantageous for researchers seeking to obtain rapid results without the need for complicated laboratory techniques. -
question:
Can nanopore sequencing detect epigenetic modifications?
répondre: Yes, nanopore sequencing has the potential to detect epigenetic modifications directly during the sequencing process. This capability stems from nanopore's ability to analyze DNA characteristics as it passes through the nanopore, which can reveal methylation patterns. Researchers are increasingly using this feature to study gene regulation and complex biological processes, enhancing our understanding of how epigenetic factors influence health and disease. -
question:
What is the typical turnaround time for results using nanopore sequencing?
répondre: Turnaround times for results using nanopore sequencing can vary based on the protocol and application, but often range from hours to a few days. The real-time data collection allows researchers to monitor sequencing in progress, which can be particularly beneficial for urgent applications, such as pathogen surveillance during outbreaks. As a result, scientists can make timely decisions, aiding in research and clinical interventions. -
question:
What types of research are ideal for nanopore sequencing?
répondre: Nanopore sequencing is ideal for various research areas, including genomics, transcriptomics, and microbial ecology. Its long-read capabilities make it particularly suitable for assembling complex genomes, studying structural variants, and resolving repetitive regions often difficult to sequence with shorter read technologies. It is widely used in evolutionary biology, cancer research, and infectious disease studies, showcasing its versatility and broad application spectrum. -
question:
What is the significance of real-time sequencing?
répondre: Real-time sequencing, a hallmark of nanopore technology, allows for immediate data acquisition and analysis as the sample is being sequenced. This timely feedback is crucial for rapid diagnostics, enabling healthcare professionals to respond quickly to emerging pathogens or genetic abnormalities. It is particularly impactful in situations where every minute counts, such as during disease outbreaks or in precision medicine applications, providing clinicians with critical information without delay. -
question:
Where can I buy Nanopore Sequencing: An Introduction in Switzerland?
répondre: You can purchase 'Nanopore Sequencing: An Introduction' on Ubuy. Ubuy offers a variety of educational and scientific materials, providing a convenient platform for obtaining this insightful book. With easy navigation and customer support, Ubuy is a reliable choice for acquiring resources related to nanopore sequencing.
Révision éditoriale
The book "Nanopore Sequencing: An Introduction" emerges as an essential resource for both novices and those with a foundational grasp of the subject. Readers have lauded its clarity and thoroughness, noting that it effectively demystifies the intricacies of nanopore sequencing technology. Many users highlight the book's ability to break down complex concepts, making it accessible for individuals seeking a deeper comprehension of the science behind this innovative sequencing method. A prevalent sentiment among reviewers is gratitude for the book's comprehensive approach, which skillfully outlines both the advantages and limitations of nanopore sequencing. This balanced perspective not only informs but also engages readers, enhancing their understanding of the technology's practical applications and potential challenges. With its well-structured content, the book is regarded as the definitive introduction to nanopore sequencing, suitable for anyone looking to gain knowledge in this rapidly evolving field. **
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Avantages
- Clear and thorough explanation of nanopore sequencing
- Accessible to both beginners and those with prior knowledge
- Comprehensive coverage of advantages and limitations
- Engaging and informative content
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Caractéristiques et avantages
- Clear explanations of the principles of nanopore sequencing
- Includes instructional materials on how to use a MinION nanopore instrument
- Serves as a textbook and lab manual at Harvard University
- Ideal for students looking to explore biology as a field of concentration
- Addresses a range of topics from electricity to informatics
- Helps students understand what their biology instrument is measuring